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3dmorph automatic analysis software  (MathWorks Inc)


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    MathWorks Inc 3dmorph automatic analysis software
    Quantitative analysis of microglial-cell morphology in the rostral ventrolateral region of the medulla. (A,D) Axioscan images of whole brainstem (AxioScan.Z1scanner, Carl Zeiss, Zaventem, Belgium) showing targeted areas from the rostral ventrolateral region of the medulla (white boxes) in one control (left) and one LPS-injected (right) lamb. Scale bar: 1000 μm. (B,E) Example of a 3D confocal image stack with full-cell segmentation automatically generated by <t>3DMorph</t> MATLAB-based script showing how quantitative morphological analysis of microglial cells (Iba1-positive cells, green) is performed. The full-cell segmentation shows remaining microglial cells after exclusion of partial cells and small processes not related to a cell body. Scale bar: 35 μm. (C,F) High magnification of the same medullary areas allows for appreciation of the morphological changes of the activated microglial cells in the LPS-injected lamb compared to the resting state in the control lamb. Qualitative analysis thus reveals a higher number of microglial cells with a larger cell-body surface, a larger total territory occupied by microglial cells, and shorter branch length in the LPS-injected lamb. Meanwhile, qualitative assessment of astrogliosis (GFAP-positive cells, red) does not suggest differences between the LPS-injected and the control lamb over the same medullary regions. Scale bar: 10 μm. (G) 3DMorph quantitative analysis of microglial-cell morphology from the above images (C,F) shows an increased average cell volume and territorial volume, as well as smaller branch length in the LPS-injected compared to the control lamb.
    3dmorph Automatic Analysis Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3dmorph automatic analysis software/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    3dmorph automatic analysis software - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Cardiorespiratory Alterations in a Newborn Ovine Model of Systemic Inflammation Induced by Lipopolysaccharide Injection"

    Article Title: Cardiorespiratory Alterations in a Newborn Ovine Model of Systemic Inflammation Induced by Lipopolysaccharide Injection

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2020.00585

    Quantitative analysis of microglial-cell morphology in the rostral ventrolateral region of the medulla. (A,D) Axioscan images of whole brainstem (AxioScan.Z1scanner, Carl Zeiss, Zaventem, Belgium) showing targeted areas from the rostral ventrolateral region of the medulla (white boxes) in one control (left) and one LPS-injected (right) lamb. Scale bar: 1000 μm. (B,E) Example of a 3D confocal image stack with full-cell segmentation automatically generated by 3DMorph MATLAB-based script showing how quantitative morphological analysis of microglial cells (Iba1-positive cells, green) is performed. The full-cell segmentation shows remaining microglial cells after exclusion of partial cells and small processes not related to a cell body. Scale bar: 35 μm. (C,F) High magnification of the same medullary areas allows for appreciation of the morphological changes of the activated microglial cells in the LPS-injected lamb compared to the resting state in the control lamb. Qualitative analysis thus reveals a higher number of microglial cells with a larger cell-body surface, a larger total territory occupied by microglial cells, and shorter branch length in the LPS-injected lamb. Meanwhile, qualitative assessment of astrogliosis (GFAP-positive cells, red) does not suggest differences between the LPS-injected and the control lamb over the same medullary regions. Scale bar: 10 μm. (G) 3DMorph quantitative analysis of microglial-cell morphology from the above images (C,F) shows an increased average cell volume and territorial volume, as well as smaller branch length in the LPS-injected compared to the control lamb.
    Figure Legend Snippet: Quantitative analysis of microglial-cell morphology in the rostral ventrolateral region of the medulla. (A,D) Axioscan images of whole brainstem (AxioScan.Z1scanner, Carl Zeiss, Zaventem, Belgium) showing targeted areas from the rostral ventrolateral region of the medulla (white boxes) in one control (left) and one LPS-injected (right) lamb. Scale bar: 1000 μm. (B,E) Example of a 3D confocal image stack with full-cell segmentation automatically generated by 3DMorph MATLAB-based script showing how quantitative morphological analysis of microglial cells (Iba1-positive cells, green) is performed. The full-cell segmentation shows remaining microglial cells after exclusion of partial cells and small processes not related to a cell body. Scale bar: 35 μm. (C,F) High magnification of the same medullary areas allows for appreciation of the morphological changes of the activated microglial cells in the LPS-injected lamb compared to the resting state in the control lamb. Qualitative analysis thus reveals a higher number of microglial cells with a larger cell-body surface, a larger total territory occupied by microglial cells, and shorter branch length in the LPS-injected lamb. Meanwhile, qualitative assessment of astrogliosis (GFAP-positive cells, red) does not suggest differences between the LPS-injected and the control lamb over the same medullary regions. Scale bar: 10 μm. (G) 3DMorph quantitative analysis of microglial-cell morphology from the above images (C,F) shows an increased average cell volume and territorial volume, as well as smaller branch length in the LPS-injected compared to the control lamb.

    Techniques Used: Injection, Generated



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    3dmorph automatic analysis software  (MathWorks Inc)
    90
    MathWorks Inc 3dmorph automatic analysis software
    Quantitative analysis of microglial-cell morphology in the rostral ventrolateral region of the medulla. (A,D) Axioscan images of whole brainstem (AxioScan.Z1scanner, Carl Zeiss, Zaventem, Belgium) showing targeted areas from the rostral ventrolateral region of the medulla (white boxes) in one control (left) and one LPS-injected (right) lamb. Scale bar: 1000 μm. (B,E) Example of a 3D confocal image stack with full-cell segmentation automatically generated by <t>3DMorph</t> MATLAB-based script showing how quantitative morphological analysis of microglial cells (Iba1-positive cells, green) is performed. The full-cell segmentation shows remaining microglial cells after exclusion of partial cells and small processes not related to a cell body. Scale bar: 35 μm. (C,F) High magnification of the same medullary areas allows for appreciation of the morphological changes of the activated microglial cells in the LPS-injected lamb compared to the resting state in the control lamb. Qualitative analysis thus reveals a higher number of microglial cells with a larger cell-body surface, a larger total territory occupied by microglial cells, and shorter branch length in the LPS-injected lamb. Meanwhile, qualitative assessment of astrogliosis (GFAP-positive cells, red) does not suggest differences between the LPS-injected and the control lamb over the same medullary regions. Scale bar: 10 μm. (G) 3DMorph quantitative analysis of microglial-cell morphology from the above images (C,F) shows an increased average cell volume and territorial volume, as well as smaller branch length in the LPS-injected compared to the control lamb.
    3dmorph Automatic Analysis Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3dmorph automatic analysis software/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    3dmorph automatic analysis software - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Quantitative analysis of microglial-cell morphology in the rostral ventrolateral region of the medulla. (A,D) Axioscan images of whole brainstem (AxioScan.Z1scanner, Carl Zeiss, Zaventem, Belgium) showing targeted areas from the rostral ventrolateral region of the medulla (white boxes) in one control (left) and one LPS-injected (right) lamb. Scale bar: 1000 μm. (B,E) Example of a 3D confocal image stack with full-cell segmentation automatically generated by 3DMorph MATLAB-based script showing how quantitative morphological analysis of microglial cells (Iba1-positive cells, green) is performed. The full-cell segmentation shows remaining microglial cells after exclusion of partial cells and small processes not related to a cell body. Scale bar: 35 μm. (C,F) High magnification of the same medullary areas allows for appreciation of the morphological changes of the activated microglial cells in the LPS-injected lamb compared to the resting state in the control lamb. Qualitative analysis thus reveals a higher number of microglial cells with a larger cell-body surface, a larger total territory occupied by microglial cells, and shorter branch length in the LPS-injected lamb. Meanwhile, qualitative assessment of astrogliosis (GFAP-positive cells, red) does not suggest differences between the LPS-injected and the control lamb over the same medullary regions. Scale bar: 10 μm. (G) 3DMorph quantitative analysis of microglial-cell morphology from the above images (C,F) shows an increased average cell volume and territorial volume, as well as smaller branch length in the LPS-injected compared to the control lamb.

    Journal: Frontiers in Physiology

    Article Title: Cardiorespiratory Alterations in a Newborn Ovine Model of Systemic Inflammation Induced by Lipopolysaccharide Injection

    doi: 10.3389/fphys.2020.00585

    Figure Lengend Snippet: Quantitative analysis of microglial-cell morphology in the rostral ventrolateral region of the medulla. (A,D) Axioscan images of whole brainstem (AxioScan.Z1scanner, Carl Zeiss, Zaventem, Belgium) showing targeted areas from the rostral ventrolateral region of the medulla (white boxes) in one control (left) and one LPS-injected (right) lamb. Scale bar: 1000 μm. (B,E) Example of a 3D confocal image stack with full-cell segmentation automatically generated by 3DMorph MATLAB-based script showing how quantitative morphological analysis of microglial cells (Iba1-positive cells, green) is performed. The full-cell segmentation shows remaining microglial cells after exclusion of partial cells and small processes not related to a cell body. Scale bar: 35 μm. (C,F) High magnification of the same medullary areas allows for appreciation of the morphological changes of the activated microglial cells in the LPS-injected lamb compared to the resting state in the control lamb. Qualitative analysis thus reveals a higher number of microglial cells with a larger cell-body surface, a larger total territory occupied by microglial cells, and shorter branch length in the LPS-injected lamb. Meanwhile, qualitative assessment of astrogliosis (GFAP-positive cells, red) does not suggest differences between the LPS-injected and the control lamb over the same medullary regions. Scale bar: 10 μm. (G) 3DMorph quantitative analysis of microglial-cell morphology from the above images (C,F) shows an increased average cell volume and territorial volume, as well as smaller branch length in the LPS-injected compared to the control lamb.

    Article Snippet: Following qualitative assessment of the number and morphology of microglial cells, a quantitative 3D-morphological analysis of microglial cells using the 3DMorph Automatic Analysis Software, a MATLAB-based script (MathWorks, Natick, MA, United States) was performed.

    Techniques: Injection, Generated